superose 12 Search Results


superose 12  (Danaher Inc)
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superose  (Danaher Inc)
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Danaher Inc superose
Superose, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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superose 12/20  (Amersham Life Sciences Inc)
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1 3 30-cm superose-12 column  (Amersham Pharmacia Biotech Ltd)
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superose 12 (hr 16/50)  (Pharmacia LKB Biotechnology Inc)
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Superose 12 (Hr 16/50), supplied by Pharmacia LKB Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fplc-superose 12  (Amersham Pharmacia Biotech Ltd)
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Amersham Pharmacia Biotech Ltd fplc-superose 12
Oocyte cAMP content and PDE3 activities in tissues from Pde3a+/+ and Pde3a–/– mice. (A) PDE activities in Pde3a+/+ and Pde3a–/– oocytes. PDE activity was assayed in oocytes from 6 Pde3A–/– and 6 Pde3a+/+ mice with [3H]-cAMP as substrate as described (22, 23) and is reported as fmol cAMP hydrolyzed/min/oocyte (mean ± SEM; n = 6). (B) cAMP content in denuded Pde3a–/– and Pde3a+/+ oocytes. As described in Methods, oocytes from 11 Pde3a+/+ and 15 Pde3a–/– mice were assayed for cAMP content, reported as fmol cAMP/oocyte. (C) PDE3 activities in heart, lung, liver, and adipose tissues from Pde3a+/+ and Pde3a–/– mice. Homogenates were prepared and PDE3 activities assayed as described in Methods. Data are means ± SEM of values (pmol cAMP hydrolyzed/min/mg protein), n = 6 mice. (D) Gel <t>filtration</t> <t>chromatography.</t> Solubilized proteins (∼4 mg), prepared from lung tissues from Pde3a+/+ or Pde3a–/– mice in buffer containing 1% NP-40 as described in Methods, were subjected to gel filtration chromatography. Left panel: protein (AU; 280 nm) (open circles, open triangles) and PDE3 activity (PDE3 cpm/10 μl) (filled circles, filled triangles) in indicated fractions from Pde3a+/+ (WT, open circles, filled circles) and Pde3a–/– (KO, open triangles, filled triangles) mice; >90% of the applied PDE3 activity was recovered in indicated fractions from +/+ mice (filled circles). Molecular weight standards: I, thyroglobulin; II, g-globulin; III, ovalbumin; IV myoglobin; V, Vit B12. Right panel: Western blots of material applied (input 10 μl) and indicated fractions (40 μl) from +/+ and –/– mice with rabbit anti-PDE3A (upper panel) and anti-PDE3B (lower panel) IgG. Recombinant rat PDE3A (r3A) was used as positive control in PDE3A Western blots.
Fplc Superose 12, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prepacked superose 12 pc 3.2/30  (Amersham Pharmacia Biotech Ltd)
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Oocyte cAMP content and PDE3 activities in tissues from Pde3a+/+ and Pde3a–/– mice. (A) PDE activities in Pde3a+/+ and Pde3a–/– oocytes. PDE activity was assayed in oocytes from 6 Pde3A–/– and 6 Pde3a+/+ mice with [3H]-cAMP as substrate as described (22, 23) and is reported as fmol cAMP hydrolyzed/min/oocyte (mean ± SEM; n = 6). (B) cAMP content in denuded Pde3a–/– and Pde3a+/+ oocytes. As described in Methods, oocytes from 11 Pde3a+/+ and 15 Pde3a–/– mice were assayed for cAMP content, reported as fmol cAMP/oocyte. (C) PDE3 activities in heart, lung, liver, and adipose tissues from Pde3a+/+ and Pde3a–/– mice. Homogenates were prepared and PDE3 activities assayed as described in Methods. Data are means ± SEM of values (pmol cAMP hydrolyzed/min/mg protein), n = 6 mice. (D) Gel <t>filtration</t> <t>chromatography.</t> Solubilized proteins (∼4 mg), prepared from lung tissues from Pde3a+/+ or Pde3a–/– mice in buffer containing 1% NP-40 as described in Methods, were subjected to gel filtration chromatography. Left panel: protein (AU; 280 nm) (open circles, open triangles) and PDE3 activity (PDE3 cpm/10 μl) (filled circles, filled triangles) in indicated fractions from Pde3a+/+ (WT, open circles, filled circles) and Pde3a–/– (KO, open triangles, filled triangles) mice; >90% of the applied PDE3 activity was recovered in indicated fractions from +/+ mice (filled circles). Molecular weight standards: I, thyroglobulin; II, g-globulin; III, ovalbumin; IV myoglobin; V, Vit B12. Right panel: Western blots of material applied (input 10 μl) and indicated fractions (40 μl) from +/+ and –/– mice with rabbit anti-PDE3A (upper panel) and anti-PDE3B (lower panel) IgG. Recombinant rat PDE3A (r3A) was used as positive control in PDE3A Western blots.
Prepacked Superose 12 Pc 3.2/30, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pharmacia superose 12 column  (Pfizer Inc)
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Pfizer Inc pharmacia superose 12 column
Oocyte cAMP content and PDE3 activities in tissues from Pde3a+/+ and Pde3a–/– mice. (A) PDE activities in Pde3a+/+ and Pde3a–/– oocytes. PDE activity was assayed in oocytes from 6 Pde3A–/– and 6 Pde3a+/+ mice with [3H]-cAMP as substrate as described (22, 23) and is reported as fmol cAMP hydrolyzed/min/oocyte (mean ± SEM; n = 6). (B) cAMP content in denuded Pde3a–/– and Pde3a+/+ oocytes. As described in Methods, oocytes from 11 Pde3a+/+ and 15 Pde3a–/– mice were assayed for cAMP content, reported as fmol cAMP/oocyte. (C) PDE3 activities in heart, lung, liver, and adipose tissues from Pde3a+/+ and Pde3a–/– mice. Homogenates were prepared and PDE3 activities assayed as described in Methods. Data are means ± SEM of values (pmol cAMP hydrolyzed/min/mg protein), n = 6 mice. (D) Gel <t>filtration</t> <t>chromatography.</t> Solubilized proteins (∼4 mg), prepared from lung tissues from Pde3a+/+ or Pde3a–/– mice in buffer containing 1% NP-40 as described in Methods, were subjected to gel filtration chromatography. Left panel: protein (AU; 280 nm) (open circles, open triangles) and PDE3 activity (PDE3 cpm/10 μl) (filled circles, filled triangles) in indicated fractions from Pde3a+/+ (WT, open circles, filled circles) and Pde3a–/– (KO, open triangles, filled triangles) mice; >90% of the applied PDE3 activity was recovered in indicated fractions from +/+ mice (filled circles). Molecular weight standards: I, thyroglobulin; II, g-globulin; III, ovalbumin; IV myoglobin; V, Vit B12. Right panel: Western blots of material applied (input 10 μl) and indicated fractions (40 μl) from +/+ and –/– mice with rabbit anti-PDE3A (upper panel) and anti-PDE3B (lower panel) IgG. Recombinant rat PDE3A (r3A) was used as positive control in PDE3A Western blots.
Pharmacia Superose 12 Column, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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superose 12 pc (3.23300 column)  (Pharmacia LKB Biotechnology Inc)
90
Pharmacia LKB Biotechnology Inc superose 12 pc (3.23300 column)
Oocyte cAMP content and PDE3 activities in tissues from Pde3a+/+ and Pde3a–/– mice. (A) PDE activities in Pde3a+/+ and Pde3a–/– oocytes. PDE activity was assayed in oocytes from 6 Pde3A–/– and 6 Pde3a+/+ mice with [3H]-cAMP as substrate as described (22, 23) and is reported as fmol cAMP hydrolyzed/min/oocyte (mean ± SEM; n = 6). (B) cAMP content in denuded Pde3a–/– and Pde3a+/+ oocytes. As described in Methods, oocytes from 11 Pde3a+/+ and 15 Pde3a–/– mice were assayed for cAMP content, reported as fmol cAMP/oocyte. (C) PDE3 activities in heart, lung, liver, and adipose tissues from Pde3a+/+ and Pde3a–/– mice. Homogenates were prepared and PDE3 activities assayed as described in Methods. Data are means ± SEM of values (pmol cAMP hydrolyzed/min/mg protein), n = 6 mice. (D) Gel <t>filtration</t> <t>chromatography.</t> Solubilized proteins (∼4 mg), prepared from lung tissues from Pde3a+/+ or Pde3a–/– mice in buffer containing 1% NP-40 as described in Methods, were subjected to gel filtration chromatography. Left panel: protein (AU; 280 nm) (open circles, open triangles) and PDE3 activity (PDE3 cpm/10 μl) (filled circles, filled triangles) in indicated fractions from Pde3a+/+ (WT, open circles, filled circles) and Pde3a–/– (KO, open triangles, filled triangles) mice; >90% of the applied PDE3 activity was recovered in indicated fractions from +/+ mice (filled circles). Molecular weight standards: I, thyroglobulin; II, g-globulin; III, ovalbumin; IV myoglobin; V, Vit B12. Right panel: Western blots of material applied (input 10 μl) and indicated fractions (40 μl) from +/+ and –/– mice with rabbit anti-PDE3A (upper panel) and anti-PDE3B (lower panel) IgG. Recombinant rat PDE3A (r3A) was used as positive control in PDE3A Western blots.
Superose 12 Pc (3.23300 Column), supplied by Pharmacia LKB Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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superosetm 12 size exclusion chromatography superose 12 10/300 gl  (Amersham Life Sciences Inc)
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Amersham Life Sciences Inc superosetm 12 size exclusion chromatography superose 12 10/300 gl
Oocyte cAMP content and PDE3 activities in tissues from Pde3a+/+ and Pde3a–/– mice. (A) PDE activities in Pde3a+/+ and Pde3a–/– oocytes. PDE activity was assayed in oocytes from 6 Pde3A–/– and 6 Pde3a+/+ mice with [3H]-cAMP as substrate as described (22, 23) and is reported as fmol cAMP hydrolyzed/min/oocyte (mean ± SEM; n = 6). (B) cAMP content in denuded Pde3a–/– and Pde3a+/+ oocytes. As described in Methods, oocytes from 11 Pde3a+/+ and 15 Pde3a–/– mice were assayed for cAMP content, reported as fmol cAMP/oocyte. (C) PDE3 activities in heart, lung, liver, and adipose tissues from Pde3a+/+ and Pde3a–/– mice. Homogenates were prepared and PDE3 activities assayed as described in Methods. Data are means ± SEM of values (pmol cAMP hydrolyzed/min/mg protein), n = 6 mice. (D) Gel <t>filtration</t> <t>chromatography.</t> Solubilized proteins (∼4 mg), prepared from lung tissues from Pde3a+/+ or Pde3a–/– mice in buffer containing 1% NP-40 as described in Methods, were subjected to gel filtration chromatography. Left panel: protein (AU; 280 nm) (open circles, open triangles) and PDE3 activity (PDE3 cpm/10 μl) (filled circles, filled triangles) in indicated fractions from Pde3a+/+ (WT, open circles, filled circles) and Pde3a–/– (KO, open triangles, filled triangles) mice; >90% of the applied PDE3 activity was recovered in indicated fractions from +/+ mice (filled circles). Molecular weight standards: I, thyroglobulin; II, g-globulin; III, ovalbumin; IV myoglobin; V, Vit B12. Right panel: Western blots of material applied (input 10 μl) and indicated fractions (40 μl) from +/+ and –/– mice with rabbit anti-PDE3A (upper panel) and anti-PDE3B (lower panel) IgG. Recombinant rat PDE3A (r3A) was used as positive control in PDE3A Western blots.
Superosetm 12 Size Exclusion Chromatography Superose 12 10/300 Gl, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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superose 12 p column  (Amersham Life Sciences Inc)
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Oocyte cAMP content and PDE3 activities in tissues from Pde3a+/+ and Pde3a–/– mice. (A) PDE activities in Pde3a+/+ and Pde3a–/– oocytes. PDE activity was assayed in oocytes from 6 Pde3A–/– and 6 Pde3a+/+ mice with [3H]-cAMP as substrate as described (22, 23) and is reported as fmol cAMP hydrolyzed/min/oocyte (mean ± SEM; n = 6). (B) cAMP content in denuded Pde3a–/– and Pde3a+/+ oocytes. As described in Methods, oocytes from 11 Pde3a+/+ and 15 Pde3a–/– mice were assayed for cAMP content, reported as fmol cAMP/oocyte. (C) PDE3 activities in heart, lung, liver, and adipose tissues from Pde3a+/+ and Pde3a–/– mice. Homogenates were prepared and PDE3 activities assayed as described in Methods. Data are means ± SEM of values (pmol cAMP hydrolyzed/min/mg protein), n = 6 mice. (D) Gel <t>filtration</t> <t>chromatography.</t> Solubilized proteins (∼4 mg), prepared from lung tissues from Pde3a+/+ or Pde3a–/– mice in buffer containing 1% NP-40 as described in Methods, were subjected to gel filtration chromatography. Left panel: protein (AU; 280 nm) (open circles, open triangles) and PDE3 activity (PDE3 cpm/10 μl) (filled circles, filled triangles) in indicated fractions from Pde3a+/+ (WT, open circles, filled circles) and Pde3a–/– (KO, open triangles, filled triangles) mice; >90% of the applied PDE3 activity was recovered in indicated fractions from +/+ mice (filled circles). Molecular weight standards: I, thyroglobulin; II, g-globulin; III, ovalbumin; IV myoglobin; V, Vit B12. Right panel: Western blots of material applied (input 10 μl) and indicated fractions (40 μl) from +/+ and –/– mice with rabbit anti-PDE3A (upper panel) and anti-PDE3B (lower panel) IgG. Recombinant rat PDE3A (r3A) was used as positive control in PDE3A Western blots.
Superose 12 P Column, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Oocyte cAMP content and PDE3 activities in tissues from Pde3a+/+ and Pde3a–/– mice. (A) PDE activities in Pde3a+/+ and Pde3a–/– oocytes. PDE activity was assayed in oocytes from 6 Pde3A–/– and 6 Pde3a+/+ mice with [3H]-cAMP as substrate as described (22, 23) and is reported as fmol cAMP hydrolyzed/min/oocyte (mean ± SEM; n = 6). (B) cAMP content in denuded Pde3a–/– and Pde3a+/+ oocytes. As described in Methods, oocytes from 11 Pde3a+/+ and 15 Pde3a–/– mice were assayed for cAMP content, reported as fmol cAMP/oocyte. (C) PDE3 activities in heart, lung, liver, and adipose tissues from Pde3a+/+ and Pde3a–/– mice. Homogenates were prepared and PDE3 activities assayed as described in Methods. Data are means ± SEM of values (pmol cAMP hydrolyzed/min/mg protein), n = 6 mice. (D) Gel filtration chromatography. Solubilized proteins (∼4 mg), prepared from lung tissues from Pde3a+/+ or Pde3a–/– mice in buffer containing 1% NP-40 as described in Methods, were subjected to gel filtration chromatography. Left panel: protein (AU; 280 nm) (open circles, open triangles) and PDE3 activity (PDE3 cpm/10 μl) (filled circles, filled triangles) in indicated fractions from Pde3a+/+ (WT, open circles, filled circles) and Pde3a–/– (KO, open triangles, filled triangles) mice; >90% of the applied PDE3 activity was recovered in indicated fractions from +/+ mice (filled circles). Molecular weight standards: I, thyroglobulin; II, g-globulin; III, ovalbumin; IV myoglobin; V, Vit B12. Right panel: Western blots of material applied (input 10 μl) and indicated fractions (40 μl) from +/+ and –/– mice with rabbit anti-PDE3A (upper panel) and anti-PDE3B (lower panel) IgG. Recombinant rat PDE3A (r3A) was used as positive control in PDE3A Western blots.

Journal:

Article Title: Cyclic nucleotide phosphodiesterase 3A-deficient mice as a model of female infertility

doi: 10.1172/JCI200421804

Figure Lengend Snippet: Oocyte cAMP content and PDE3 activities in tissues from Pde3a+/+ and Pde3a–/– mice. (A) PDE activities in Pde3a+/+ and Pde3a–/– oocytes. PDE activity was assayed in oocytes from 6 Pde3A–/– and 6 Pde3a+/+ mice with [3H]-cAMP as substrate as described (22, 23) and is reported as fmol cAMP hydrolyzed/min/oocyte (mean ± SEM; n = 6). (B) cAMP content in denuded Pde3a–/– and Pde3a+/+ oocytes. As described in Methods, oocytes from 11 Pde3a+/+ and 15 Pde3a–/– mice were assayed for cAMP content, reported as fmol cAMP/oocyte. (C) PDE3 activities in heart, lung, liver, and adipose tissues from Pde3a+/+ and Pde3a–/– mice. Homogenates were prepared and PDE3 activities assayed as described in Methods. Data are means ± SEM of values (pmol cAMP hydrolyzed/min/mg protein), n = 6 mice. (D) Gel filtration chromatography. Solubilized proteins (∼4 mg), prepared from lung tissues from Pde3a+/+ or Pde3a–/– mice in buffer containing 1% NP-40 as described in Methods, were subjected to gel filtration chromatography. Left panel: protein (AU; 280 nm) (open circles, open triangles) and PDE3 activity (PDE3 cpm/10 μl) (filled circles, filled triangles) in indicated fractions from Pde3a+/+ (WT, open circles, filled circles) and Pde3a–/– (KO, open triangles, filled triangles) mice; >90% of the applied PDE3 activity was recovered in indicated fractions from +/+ mice (filled circles). Molecular weight standards: I, thyroglobulin; II, g-globulin; III, ovalbumin; IV myoglobin; V, Vit B12. Right panel: Western blots of material applied (input 10 μl) and indicated fractions (40 μl) from +/+ and –/– mice with rabbit anti-PDE3A (upper panel) and anti-PDE3B (lower panel) IgG. Recombinant rat PDE3A (r3A) was used as positive control in PDE3A Western blots.

Article Snippet: Supernatants (total tissue lysates) were used for PDE assays ( 35 ) or gel filtration chromatography (FPLC-superose 12; AKTA FPLC System; Amersham-Pharmacia Biotech Inc., Piscataway, New Jersey, USA).

Techniques: Activity Assay, Filtration, Chromatography, Molecular Weight, Western Blot, Recombinant, Positive Control